Simmons Citrate Agar M099

วัตถุประสงค์การใช้งาน

Simmons Citrate Agar is recommended for differentiation the members of Enterobacteriaceae on the basis of citrate

utilization from clinical and non clinical samples. .

Composition**

Ingredients Gms / Litre

Magnesium sulphate 0.200

Ammonium dihydrogen phosphate 1.000

Dipotassium phosphate 1.000

Sodium citrate 2.000

Sodium chloride 5.000

Bromothymol blue 0.080

Agar 15.000

Final pH ( at 25°C) 6.8±0.2

**Formula adjusted, standardized to suit performance parameters

วิธีการเตรียม

Suspend 24.28 grams in 1000 ml distilled water. Heat, to boiling, to dissolve the medium completely. Mix well and distribute

in tubes or flasks. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Precaution: Before using water, ensure pH of water is 6.5 to 7.0.Initial colour of the medium may deviate from expected

colour, if the above precaution is ignored.