MacConkey Sorbitol Agar (Sorbitol Agar) M298

วัตถุประสงค์การใช้งาน

MacConkey Sorbitol Agar is used for isolation and identification of enteropathogenic Escherichia coli strains associated

with infant diarrhea.

Composition**

Ingredients Gms / Litre

Peptone 17.000

Proteose peptone 3.000

D-Sorbitol 10.000

Bile salts mixture 1.500

Sodium chloride 5.000

Neutral red 0.030

Crystal violet 0.001

Agar 13.500

Final pH ( at 25°C) 7.1±0.2

**Formula adjusted, standardized to suit performance parameters

วิธีการเตรียม

Suspend 50.03 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving

at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING. Cool to 45-50°C and pour into sterile Petri plates