Staphylococcus Agar No.110 M521

วัตถุประสงค์การใช้งาน

(Gelatin Mannitol Salt Agar)

Staphylococcus Agar No.110 is used as a selective medium for the isolation and testing of pathogenic Staphylococci.

Composition**

Ingredients Gms / Litre

Casein enzymic hydrolysate 10.000

Yeast extract 2.500

Gelatin 30.000

Lactose 2.000

D-Mannitol 10.000

Sodium chloride 75.000

Dipotassium phosphate 5.000

Agar 15.000

Final pH ( at 25°C) 7.0±0.2

**Formula adjusted, standardized to suit performance parameters

วิธีการเตรียม

Suspend 149.5 grams in 1000 ml of distilled water. Mix thoroughly. Heat, to boiling, to dissolve the medium completely.

Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Resuspend the precipitate by gentle agitation to avoid

bubbles and pour the plates while the medium is hot. Alternatively, cool the medium to 45 - 50°C and add blood or egg yolk

if desired. This medium may also be used without sterilization; it should be boiled for 5 minutes and used at once