Streptococcus Lactis Differential Agar Base M925

วัตถุประสงค์การใช้งาน

Streptococcus Lactis Differential Agar Base is used for differentiation of citrate-utilizing lactic streptococci - Lactococcus

lactis (Streptococcus lactis) subspecies diacetylactis from citrate non-utilizing Lactococcus lactis (Streptococcus lactis)

and Lactococcus lactis (Streptococcus lactis) subspecies cremoris .

Composition**

Ingredients Gms / Litre

Nonfat (skim) milk 10.000

Peptonized milk 2.500

Dextrose 5.000

Agar 15.000

Final pH ( at 25°C) 6.6±0.2

**Formula adjusted, standardized to suit performance parameters

วิธีการเตรียม

Suspend 32.5 grams in 1000 ml distilled water. Heat to boiling with stirring to dissolve the medium completely. Sterilize by

autoclaving at 10 lbs pressure (115°C) for 12 minutes. Cool to 45°C and aseptically add (30 minutes steam-sterilized solutions)

10 ml of 10% potassium ferricyanide and 10 ml of citrate solution containing 0.25 g ferric citrate and 0.25 gram sodium citrate.

Gently mix and pour into the sterile Petri plates. Dry the plates in dark for 24 hours at 30°C