Chocolate agar Base is recommended for the isolation of Neisseria gonorrhoeae from chronic and acute cases ofgonococcal infections.

Proteose peptone 20.000

Dextrose (Glucose) 0.500

Sodium chloride 5.000

Disodium phosphate 5.000

Agar 15.000

Final pH ( at 25°C) 7.3±0.2

**Formula adjusted, standardized to suit performance parameters

1.Suspend 45.5 grams in 495 ml distilled water.
2.Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at

15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C.
3. Aseptically add equal amount (495 ml)of sterile 2% Haemoglobin solution (FD022). Also add the contents or one vial of Yeast Autolysate Supplement (FD027) or Vitamino Growth Supplement

(FD025) reconstituted as directed.
4. Mix well before pouring. When single strength medium is desired, suspend 45.5 grams in

 1000 ml distilled water