Columbia Blood Agar Base is used as an efficient base for preparation of blood agar, chocolate agar and for preparation of

various selective and identification media and isolation of organisms from clinical and non clinical samples.

Peptone, special 23.000

Corn starch 1.000

Sodium chloride 5.000

Agar 15.000

Final pH ( at 25°C) 7.3±0.2

**Formula adjusted, standardized to suit performance parameters

1.Suspend 44.0 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely.
2.Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C before adding heat sensitive compounds.