Nutrient Broth is used for the general cultivation of less fastidious microorganisms , can be enriched with blood or other

biological fluids.

Peptone 5.000

Sodium chloride 5.000

HM peptone B# 1.500

Yeast extract 1.500

Final pH ( at 25°C) 7.4±0.2


วิธีการเตรียม
Suspend 13.0 grams in 1000 ml distilled water. Heat, if necessary, to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.