Phenylalanine Agar M281

วัตถุประสงค์การใช้งาน

Phenylalanine Agar is used for the differentiation of Proteus and Providencia group of organisms from other members

of Enterobacteriaceae on the basis of their ability to form phenyl pyruvic acid from phenylalanine.

Composition**

Ingredients Gms / Litre

Yeast extract 3.000

Sodium chloride 5.000

DL-Phenylalanine 2.000

Disodium phosphate 1.000

Agar 15.000

Final pH ( at 25°C) 7.3±0.2

**Formula adjusted, standardized to suit performance parameters

วิธีการเตรียม

Suspend 26 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense in tubes and sterilize

by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubed medium to cool in a slanting position.