Antibiotic Assay Medium No. 3 (Assay Broth) M042
Ingredients Gms / Litre
Peptone 5.000
HMpeptone B # 1.500
Yeast extract 1.500
Dextrose (Glucose) 1.000
Sodium chloride 3.500
Dipotassium hydrogen phosphate 3.680
Potassium dihydrogen phosphate 1.320
Final pH ( at 25°C) 7.0±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract

Directions
Suspend 17.5 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Advice: Recommended for the microbiological assay of Amikacin, Capreomycin, Chlortetracycline,Chloramphenicol, Cycloserine,Demeclocycline,Dihydrostreptomycin, Doxycycline, Gentamicin, Gramicidin, Kanamycin, Methacycline, Neomycin, Novobiocin, Oxytetracycline, Rolitetracycline, Streptomycin, Tetracycline, Tobramycin, Trolendomycin and Tylosin according to official methods.